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How to test HEROIN (opioids)?
Home›Magazine›Testing›How to test HEROIN (opioids)?
Testing Fent, Fentanyl, Froehde, Heroin, Marques, Marquis, Mecke, Opiates, Opio, Opioids, Strips, Test strip, Test strips

29 September 2020

How to test Heroin (Opioids)?

How to test Heroin (opioids): if you intend to use a psychoactive substance first you need a test kit to check purity. Pure heroin can’t be identified by appearance. Check your sample with a single or multi-use heroin test kit. 

Heroin tester

Single use heroin tester available with free shipping. This test gives the least amount of information and should be used with extra caution. Read about more advanced tools further down.
  • Single-use Heroin Reagent Test

    PRO Test Kit for Opioids Identification

    Rated 5.00 out of 5
    €3,44 Add to cart

Recommended heroin testing kits

Recommended multi-use Heroin Reagent Test Kit includes reagents Marquis, Mecke, Froehde and fentanyl test strips. Reagents are required to identify heroin or other opioids, while fentanyl test kit is irreplacable to detect presene of fentanyl, even if mixed in and present in trace amounts. To discover full ingredients and estimate heroin % pair reagents with the Full TLC Purity Test Kit. To detect fentanyl use the fent strips.

STEP ONE How to identify heroin?

How to use reagent tests?

Instrukcja testowania DMT

Watch how to test heroin with reagents:

Watch how to test heroin with reagents:

TL;DR: Below is an image with a quick cheat sheet how to use the Heroin Test Kit and further down you can check out more detailed guide how to test heroin. Use the heroin reagent test chart to see how to use Marquis reagent, Mecke and Froehde. Check the expected results.

Heroin test results chart:

TEST Heroin
MARQUIS Reddish purple
MECKE Green-blue
MANDELIN Red > Dark brown
LIEBERMANN Purple / black
FROEHDE Purple red > black
EHRLICH No color change
HOFMANN No color change
SIMON’S No blue color change
ROBADOPE No pink color change
MORRIS Green
ZIMMERMANN No color change
CANNABIS (4-AP) No color change

 

Opioids Test Color Chart

Heroin reagent test chart 

Heroin color chart

All results are available in our booklet and app.

What are heroin test kit reagents used for?

SAMPLE RECOMMENDED HEROIN REAGENT TESTS
HEROIN / CODEINE / O-DSMT /
 2C-B /  MDMA / MDA / METH/AMPHETAMINE 
  • Marquis
  • Mecke
  • Froehde
  • single-use heroin test kit
  • multiple-use heroin test kit

Reagent tests indicate presence of a substance, therefore can either help confirm presence of the expected compound or help rule out certain cuts.

  • Marquis – main test for most powders, secondary test for ketamine, mephedrone and psychedelics
  • Mecke – main test for most powders, secondary test for cocaine, ketamine and mephedrone
  • Froehde – main test for most powders, secondary test for ketamine and amphetamines, long shelf life
  • Single use heroin test kit includes Froehde reagent
  • Multi use heroin reagent test kit includes reagents Marquis, Mecke and Froehde

Froehde reagent is the main test for heroin as it can tell it apart from codeine. It should not be used alone, requires Marquis and Mecke to confrm presence of specific opioids.

Testing codeine with Marquis reagent, Mecke and Froehde

Negative heroin test kit results

Testing codeine with Marquis reagent, Mecke and Froehde

What is fentanyl?

Fentanyl is an extremely potent synthetic opioid with derivatives often from 50 to 100 times stronger than heroin. It is mainly responsible for the opioid epidemic overdoses. Fentanyl can be lethal even in doses invisible to a bare eye. It is often found in counterfeit medicine, opioid and non-opioid research chemicals or even common street drugs like cocaine. 
When fentanyl is mixed with heroin, cocaine or other substances, it is never evenly mixed. Powder on one side of a baggy can contain no fentanyl, while powder from the other side might contain a lethal dose. It is extremely important to always test each and every dose intended for consumption.

Questions?

  • What is Opioids Test Kit for?

    Opioids Test Kit includes 4 fentanyl testing strips and 3 reagents (Marquis, Mecke, Froehde) best for heroin or other opioids, but also suitable for meth/amphetamines, MDxx and more. It is used to positively identify heroin, morphine, codeine, oxycodone and to rule out U-47700, U-49900, fentanyl or different, less predictable, less desirable, highly toxic dangerous drugs. Multiple reagents are required to detect adulterants and increase analysis precision.

    Reagent tests can indicate PRESENCE, but not PURITY or QUANTITY. Mixtures can be detected with reagents only if adulterant causes a darker color change than the expected substance. To detect all ingredients and estimate their amount pair reagents with a TLC purity test kit.

  • What is included in Opioids Reagent Test Kit?

    Opioids Reagent Test Kit includes 4 fentanyl test strips, 3 reagents (Froehde, Marquis, Mecke), absorbent vermiculite, a porcelain reaction plate, a spatula, gloves, instructions and app for all test kits and over 800 substances. Every bottle contains 10 ml of crystals, 100 tests of each in total. High quality HDPE and brown amber glass protect reagents from light, plastic and moisture to ensure prolonged shelf life. Child-proof caps guarantee 0 spills.

  • What is each reagent test for?

    Opioids Test Kit is best for heroin or other opioids but is also suitable for meth/amphetamines, MDxx and more.

    Reagent test results chart – which reagent reacts with which substance

    • Cannabis (4-AP) – main test for THC / CBD, helps rule out synthetic cannabinoids
    • Ehrlich – main test for all indoles, e.g. lysergamides and tryptamines but also 5-HTP and more, secondary test for cocaine
    • Hofmann – main test for lysergamides and tryptamines, more precise but slower than Ehrlich
    • Froehde – main test for most powders, secondary test for ketamine and amphetamines, long shelf life
    • Liebermann – main test for most powders, secondary test for cocaine (detects levamisole)
    • Marquis – main test for most powders, secondary test for ketamine, mephedrone and psychedelics
    • Mecke – main test for most powders, secondary test for cocaine, ketamine and mephedrone
    • Morris – main test for cathinones, cocaine and ketamine, can tell apart some novel dissociatives
    • Robadope – main test for primary amines, e.g. MDA, amphetamine or PMA
    • Simon – main test for secondary amines, e.g. MDMA, meth, mephedrone, methylone or PMMA
    • Zimmermann / Beam – main test for cathinones and benzodiazepines in geneal, detects also CBD

    Fentanyl Test Strip to detect fentanyl, even if it is mixed in another substance.

    Reagents can indicate PRESENCE, but not PURITY or QUANTITY. Adulterants can be detected with reagents only if they cause a darker color change than the expected substance. To detect all ingredients and estimate their amount pair reagents with a TLC purity test kit.

  • How to use Opioids Reagent Test Kit?

    GET ALL TOOLS

    First, prepare a clean white testing surface – ceramic or glass is the best, e.g. bottom of a mug or a plate. Get your sample, reagents, a sharp tool / scoop and paper towels.

    PREPARE SAMPLE, AT LEAST THIS BIG (MAX 5 MG) -> ●   

    If testing a pill, powder or crystal, crush it finely. If testing a liquid, dry one drop on paper or clean ceramic surface. If testing a blotter or gel tab, cut off at least 1/8th (a corner). A bigger sample can cause a stronger reaction. You need a new sample for each test.

    ADD PROPORTIONAL AMOUNT OF REAGENT

    Shake bottles hard before use, then tap firmly to shake down any reagent stuck in cap. Do not let a bottle touch sample or you will ruin the rest of the reagent. Use just enough reagent to cover a sample. If using crystals avoid air pockets by flattening crystals onto sample using a clean tool. Do not combine reagents except for „A” and „B” bottles. If using Morris reagent, mix it with a clean spatula or toothpick for 30 seconds.

    OBSERVE FOR UP TO 2 MINUTES*

    Disregard any significant colour change after 1-2 minutes. Fumes can occur. Do not overinterpret your results, consider that color change reaction is always fastest and most vivid in the part where biggest surface of reagent touches sample, compared to its edges. Over time reaction will degrade due to air contact. Take photos.

    COMPARE RESULTS

    Compare color change with instructions. Reagents can indicate PRESENCE, but not PURITY or QUANTITY. Adulterants can be detected with reagents only if they cause a darker color change than the expected substance. To detect all ingredients and estimate their amount pair reagents with a TLC purity test kit.

    Clean used reagent tools / testing surface with lots of running water and detergent, avoid contamination.

  • How to interpret Opioids Test Kit results?
    Instructions for testing Opioids
    Opioids Test Kit Chart


    Expected opioids test kit results:

    • Marquis reagent – purple (heroin)
    • Mecke reagent – green (heroin)
    • Froehde reagent – purple (heroin)

    CLICK BELOW TO VIEW PRO TEST REAGENT TESTING BOOKLET IN FULL RESOLUTION

    PRO Test reagent testing booklet

     CHECK ALL REAGENT TEST REACTIONS IN RESULTS APP AT PROTESTKIT.EU/RESULTS

  • How to handle Opioids Test Kit?

    Drug testing kits contain toxic and/or corrosive chemicals, strong enough to burn skin and clothing. Keep out of eyes and mouth. Keep away from children and animals. Always use in a well-ventilated area. Wear gloves (supplied) when handling the bottle and cap. Wear protective eyewear. Never point towards face etc. If you get some on you/clothing, then immediately wash with lots of soap and water. Dispose of any unwanted reagent with running water, ventilate. Store reagents standing up and away from heat in a cool, dark place. The average shelf life of 2 years can be significantly extended by storing in a fridge or freezer (warm up to room temperature before use).

    Reagents deteriorate with time or due to contact with air, especially if exposed to sunlight or not refrigerated. If you are uncertain whether the reagent will work, test it with an already confirmed sample.

    STORAGE

    • Absorbent vermiculite can be thrown away – it is only there to secure the reagents in transit.
    • Keep away from heat and sunlight.
    • Store bottles tightly closed and keep granules out of the cap (“crunchy lid syndrome”).
    • Tests have an average shelf life of 2 years, depending on storage conditions. A fridge or freezer extends it considerably – warm to room temperature before use.

    HANDLING

    • Always wear gloves; consider protective eyewear and a mask.
    • Only test in a well-ventilated area, and avoid the fumes.
    • Have running water available to clean up after testing.
    • Never open more than one bottle at once.
    • Warm reagents to room temperature before use.
    • Avoid contamination: wash and fully dry all accessories after use.
    • Use paper towels to wipe a used plastic spatula clean straight away.
    • Do not leave reagents unattended, and keep them away from children and animals.

    CRYSTALLINE REAGENTS IN GLASS BOTTLES

    • Shake the reagent bottle for 5-10 seconds right before use.
    • Tap the bottle firmly to bring crystals down from the cap.
    • To add crystalline reagent, pour it out – tap the bottle or use a spatula.
    • Flatten the solid reagent on top of the sample for the best result (optional).
    • Wipe the cap and neck to avoid a “crunchy lid” and protect the cap seal.

    DISPOSAL

    • Wash used or spilled reagent away with plenty of water and soap.
    • Dispose of product contents or contaminated materials according to local procedures for hazardous waste.
  • How to test opioids for fentanyl?

    Use fentanyl test strips (a fentanyl test kit)

    Fentanyl test strip instructions – how to use fentanyl test strips

    METHOD 1   

    If testing a benzo/opioid pill, crush one in a plastic baggie and dump out the powder.  Then add 10 drops of water into the baggie and mix well. Dip the test strip no higher than the thick line. Wait 15 seconds. Put the strip away and check in 5 minutes.

    METHOD 2

    Mix sample in the ratio of 1 mg of sample per 2 ml of water. Dip the test strip no higher than the thick line. Wait 15 seconds. Put the strip away and check in 5 minutes.

    COMPARE RESULTS

    1 line = fentanyl, 2 lines = no fentanyl. Faint lines also count. Ignore fentanyl tester results after 5 minutes.

    To detect all ingredients and estimate their amount pair reagents with a TLC purity test kit.

  • More questions?

    Click here to see our main FAQ on the home page.

STEP TWO How to test heroin purity %?

To discover all adulterants and estimate quantity pair reagent tests with a TLC (Thin Layer Chromatography) Purity Test Kit. TLC purity tests alone don’t identify substances and are best paired with at least 1-2 reagent tests. Thin Layer Chromatography is one of classic substance analysis methods. It works by separating different compounds depending on their solubility in chosen TLC testing liquid (eluent). To precisly estimate heroin purity pair a reagent test kit with the Full TLC Purity Test Kit.

How to test quality of heroin?

Qualitative TLC:

TLC test works by separating substances present in a sample, each detected spot indicates a different substance. 

  • You can use an already confirmed sample for reference – if both compounds show up at the same height on the TLC “card” that indicates they can be the same substance.
  • You can use any substance for reference after establishing their relation – if substance X shows up twice as high on the same testing “card” as substance Y, you can expect them to keep that ratio.
TLC Cocaine Purity Test

TLC Cocaine Purity Test quantity scale

Reference adjusted from 66% pure cocaine with coffeine and ethenzamide. Due to UV-C light photo might not display correctly.
Range of MDMA spot sizes from 10% to 100%

TLC MDMA Purity Test quantity scale

Reference results ranging from 10% up to 100%, adjusted from lab confirmed pure MDMA hydrochloride sample

How to test quality of heroin?

Semi-quantitative TLC:

TLC helps estimate quantity (quantity). The bigger the size of the detected spot, the bigger the amount of measured substance. Note that different substances need different calibrated rulers and often different testing liquids (eluents).

  • One spot indicates a pure substance. If your sample fully dissolved in testing liquid and only one spot was detected on TLC then your substance is pure. Trace amounts of impurities might not be detected, do not rely on TLC for fentanyl testing – get also fentanyl test strips.

How to use TLC purity tests?

TLC pictograms

Watch how to test heroin with TLC:

Pair with a reagent test kit to identify substances. Use both kits separately.

Watch how to test heroin with TLC:

Pair with a reagent test kit to identify substances. Use both kits separately.

Questions?

  • What is Full TLC Purity Test Kit for?

    Full TLC Purity Test Kit includes best testing liquid and accessories for cannabinoids, cocaine, MDMA 2C-B but also suitable for ketamine, meth/amphetamine, opioids, phenethylamines, tryptamines and more. It is used to discover full ingredients and amount (%) of CBD, THC, MDMA, cocaine or 2C-B, DMT, LSD, ketamine, meth/amphetamine, heroin and to rule out caines, caffeine, cathinones, levamisol, PMA/PMMA or different, less predictable, less desirable, highly toxic dangerous drugs. Additional multi-reagent test kit is required to identify ingredients and increase analysis precision.

    TLC purity test kits can detect NUMBER and AMOUNT of substances, but not their TYPE. Pair TLC test kit with reagent test kit to indentify ingredients. Reagents alone detect mixtures only if adulterant causes a darker color change than the expected substance.

  • What is included in Full TLC Purity Test Kit?

    Full TLC Purity Test Kit includes instructions, gloves, 4 vials, 5 testing “cards”, jar, pipette, testing liquid (40 ml), 20 glass tubes, UV-C flashlight (4 AA batteries required, not included) and printed % scales for CBD, THC, cocaine and MDMA. Each 1 “card”, 4 glass tubes and 4 ml of testing liquid equal 4 tests. Testing “card” can be reused untill fully used. Glass and metal protect testing liquid from air and moisture to ensure prolonged shelf life. High-quality caps and lid guarantee 0 spills. 

    TLC purity test kit for MDMA includes 3 testing liquid for MDMA, MDA, 2C-B, DXM, DMT, LSD, ketamine, caines, cannabinoids, caffeine, codeine, mCPP, meth/amphetamine, metoclopramide, paracetamol and other. It is best to use this test kit for cystals, powders, pills or plants.

  • How to use Full TLC Purity Test Kit?

    GET ALL TOOLS

    First, prepare a clean and well lit testing surfach. Get your sample, spatula, gloves, plastic vial, testing liquid, pipette, pencil, glass tube, wrapped esting cards, jar and UV-C flashlight. Next, carefully holding a testing “card” by only top edges mark 4 dots 1 cm apart from card’s bottom, sides and each other.

    PREPARE SAMPLE

    If testing a pill, powder or crystal, crush it finely. Weigh sample in a plastic vial and dissolve in testing liquid (or other solvent) using ratios mentioned in the booklet. Shake to dissolve. To big or too small sample can cause an inconclusive result.

    ADD 1 MICROLITER TO CARD 

    In case of sediment wait a minute for it to fall down. Under strong light submerge end of glass tube to fill it with dissolved sample. Gently touch one of the marked dots on Testing Card to discharge sample and mark the spot with pencil.

    INSERT CARD INTO JAR

    Add 2 ml of testing liquid inside jar and carefully, paying attention to not touch the white side of Testing Card with sample, insert it inside jar. Close the jar and observe for about 25 minutes, until Testing Card almost fully soaks – but not any longer. Remove Testing Card from jar, wait for it to dry.

    COMPARE RESULTS

    Use UV-C flashlight to reveal results. Spots on Testing Card revealed by UV-C light circle using pencil. Each spot indicates a separate substance.  TLC purity test kits can detect NUMBER and AMOUNT of substances, but not their TYPE. Pair TLC test kit with reagent test kit to indentify ingredients. Reagents alone detect mixtures only if adulterant causes a darker color change than the expected substance.

    To reuse Testing Card do not apply reagent tests on it.

  • How to interpret results?

     

    CLICK BELOW TO VIEW TLC PURITY TEST KIT BOOKLET IN FULL RESOLUTION

    TLC purity test kit instructions

  • How to handle TLC tests?

    Testing liquids contain toxic and/or corrosive chemicals, strong enough to burn skin and clothing. Keep out of eyes and mouth. Keep away from children and animals. Always use in a well-ventilated area. Wear gloves (supplied) when handling the bottle and cap. Wear protective eyewear. Never point towards face etc. If you get some on you/clothing, then immediately wash with lots of soap and water. Dispose of any unwanted reagent with running water, ventilate. Store away from heat in a cool, dark place. TLC tests do not expire if stored correctly.

  • What TLC testing liquids are available?

    We recommend to get spare testing liquid to save on shipping.
    Note: you can use any solvent to dissolve your sample, e.g. ethanol 

    Check which Testing Liquid is best for testing which substances:
    • Primary Testing Liquid for – alkaloids, phenetylamines (amphetamine, MDMA, 2C-B, etc.), indolamines (LSD, DMT, etc.), tryptamines, derivatives and opiates
      • e.g. 2C-B, DMT, LSD, heroin, DXM, MDMA, meth/amphetamine, mCPP, metoclopramide, codeine, caffeine, acetaminophen, procaine, ketamine
    • Testing Liquid for Cocaine Purity Test – ketamine, cocaine, caffeine, levamisole, lidocaine, procaine, tetracaine, benzocaine, acetaminophen
    • Testing Liquid for Cannabinoid Test – for testing all natural cannabinoids and cannabis products
  • What is each colorimetric reagent test for?

    Full TLC Purity Test Kit is best paired with Full Reagent Test Kit, recommended for 800+ substances.

    Reagent test results chart – which reagent reacts with which substance

    • Cannabis (4-AP) – main test for THC / CBD, helps rule out synthetic cannabinoids
    • Ehrlich – main test for all indoles, e.g. lysergamides and tryptamines but also 5-HTP and more, secondary test for cocaine
    • Hofmann – main test for lysergamides and tryptamines, more precise but slower than Ehrlich
    • Froehde – main test for most powders, secondary test for ketamine and amphetamines, long shelf life
    • Liebermann – main test for most powders, secondary test for cocaine (detects levamisole)
    • Marquis – main test for most powders, secondary test for ketamine, mephedrone and psychedelics
    • Mecke – main test for most powders, secondary test for cocaine, ketamine and mephedrone
    • Morris – main test for cathinones, cocaine and ketamine, can tell apart some novel dissociatives
    • Robadope – main test for primary amines, e.g. MDA, amphetamine or PMA
    • Simon – main test for secondary amines, e.g. MDMA, meth, mephedrone, methylone or PMMA
    • Zimmermann / Beam – main test for cathinones and benzodiazepines in geneal, detects also CBD

    Fentanyl Test Strip to detect fentanyl, even if it is mixed in another substance.

    Reagents can indicate PRESENCE, but not PURITY or QUANTITY. Adulterants can be detected with reagents only if they cause a darker color change than the expected substance. To detect all ingredients and estimate their amount pair reagents with a TLC purity test kit.

  • How to detect fentanyl?

    Use fentanyl test strips (a fentanyl test kit)

    Fentanyl test strip instructions – how to use fentanyl test strips

    METHOD 1   

    If testing a benzo/opioid pill, crush one in a plastic baggie and dump out the powder.  Then add 10 drops of water into the baggie and mix well. Dip the test strip no higher than the thick line. Wait 15 seconds. Put the strip away and check in 5 minutes.

    METHOD 2

    Mix sample in the ratio of 1 mg of sample per 2 ml of water. Dip the test strip no higher than the thick line. Wait 15 seconds. Put the strip away and check in 5 minutes.

    COMPARE RESULTS

    1 line = fentanyl, 2 lines = no fentanyl. Faint lines also count. Ignore fentanyl tester results after 5 minutes.

    To detect all ingredients and estimate their amount pair reagents with a TLC purity test kit.

  • More questions?

    Click here to see our main FAQ on the home page.

How to test heroin for fentanyl?

Use fentanyl test strips (a fentanyl test kit)

Fentanyl test strip instructions – how to use fentanyl test strips

METHOD 1   

If testing a benzo/opioid pill, crush one in a plastic baggie and dump out the powder.  Then add 10 drops of water into the baggie and mix well. Dip the test strip no higher than the thick line. Wait 15 seconds. Put the strip away and check in 5 minutes.

METHOD 2

Mix sample in the ratio of 1 mg of sample per 2 ml of water. Dip the test strip no higher than the thick line. Wait 15 seconds. Put the strip away and check in 5 minutes.

COMPARE RESULTS

1 line = fentanyl, 2 lines = no fentanyl. Faint lines also count. Ignore fentanyl tester results after 5 minutes.

To detect all ingredients and estimate their amount pair reagents with a TLC purity test kit.

Check all PRO Test guides how to test drugs.

 

No test result can tell you a substance is safe. No substance is 100% safe.This article is published for harm reduction purposes. It does not encourage the use of psychoactive substances and does not replace medical advice.

How to read the result

A positive result is a colour change resembling one shown in the instructions. It does NOT indicate quantity, purity or safety. Adulterants often do not react at all – try to detect them using different reagents. A negative result is a colour change that differs from the instructions: your sample is adulterated, discard it.

Harm reduction

There is no such thing as fully safe drug use, but harm reduction rules can help make psychoactive substances safer. Always do an “allergy test” – with a substance you do not know yet, start with 1/3 of a dose or less. Always use the smallest dose needed for the effect you want. Never share equipment, and do not use dirty objects such as banknotes (infection risk). Do not redose, at least not for 2-3 hours. Avoid mixing psychoactive substances.

What changed since this guide was written: nitazenes and xylazine

Reagent testing was designed around a supply that has since changed. Two additions matter more than anything a colour chart can show you, and neither is reliably detected by reagents.

  • Nitazenes (etazene and its analogues) are synthetic opioids now appearing in heroin, in counterfeit benzodiazepine pills and in samples sold as other substances entirely. Some are more potent than fentanyl. Reagents will not tell you they are there. Use nitazene test strips, and read How to detect nitazenes?
  • Xylazine is a veterinary sedative increasingly mixed into opioid supplies. It is not an opioid, so naloxone will not reverse its effects, though naloxone should still be given because xylazine is almost always found alongside an opioid. Use xylazine test strips, and read Test strips for xylazine.
  • Fentanyl remains the most important single check. Use fentanyl test strips.

All three can be checked with one product: Test Strips for Fentanyl, Nitazenes and Xylazine. Carry naloxone, never use alone, and start with a fraction of your usual amount when the source is new.

How to test METHAMPHETAMINE? How to test COCAINE?

Single-use tests

  • “Is this a drug?” Test Kit (3 tests)
  • EZ Test for Cocaine Quantity
  • EZ Test for MDMA Quantity
  • Fentanyl Test Strip
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